Functional expression of the alpha 1 subunit of the AMPA-selective glutamate receptor channel, using a baculovirus system

Biochem Biophys Res Commun. 1991 Dec 16;181(2):756-63. doi: 10.1016/0006-291x(91)91255-b.

Abstract

Using a baculovirus expression vector system, the alpha 1 subunit of the mouse AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-selective glutamate receptor channel was expressed in insect Spdoptera frugiperda cells. Binding studies using [3H]AMPA showed that insect cells infected with the recombinant virus expressed approximately 1.8 x 10(5) binding sites per cell on their surface. The ligand binding characteristics of the receptors expressed in insect cells were examined. The baculovirus-insect cell expression system affords high-efficiency expression of the receptor in sufficient amounts to permit structural and functional analyses.

MeSH terms

  • Animals
  • Baculoviridae / genetics*
  • DNA, Recombinant
  • Gene Expression
  • Genetic Vectors
  • Glutamates / metabolism
  • Glutamates / pharmacology
  • Glutamic Acid
  • Ibotenic Acid / analogs & derivatives*
  • Ibotenic Acid / metabolism
  • Ibotenic Acid / pharmacology
  • Insecta / genetics
  • Insecta / metabolism
  • Ion Channels / metabolism*
  • Kainic Acid / metabolism
  • Kainic Acid / pharmacology
  • Kinetics
  • Mice
  • Quisqualic Acid / pharmacology
  • Receptors, Glutamate
  • Receptors, Neurotransmitter / drug effects
  • Receptors, Neurotransmitter / genetics*
  • Receptors, Neurotransmitter / metabolism
  • Transfection
  • alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid

Substances

  • DNA, Recombinant
  • Glutamates
  • Ion Channels
  • Receptors, Glutamate
  • Receptors, Neurotransmitter
  • Ibotenic Acid
  • Glutamic Acid
  • alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
  • Quisqualic Acid
  • Kainic Acid