Abstract
The electrophysiological properties of the two major glial cell types in cultures from the regenerating goldfish optic nerve/tract were studied with patch-clamp techniques. Spindle-shaped cells express myelin proteins. These oligodendrocyte-like cells possess outwardly rectifying currents, do not show glutamate activated currents and are rarely electrically coupled to neighboring cells. Cells of epitheloid morphology probably represent astrocytes. They are GFAP-positive and do not exhibit myelin proteins. These cells have glutamate activated currents, display a linear current to voltage relationship and are extensively electrically coupled thus displaying properties similar to mammalian astrocytes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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4-Aminopyridine / pharmacology
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Animals
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Cells, Cultured
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Electrophysiology / methods
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Glial Fibrillary Acidic Protein / analysis
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Glutamates / pharmacology
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Glutamic Acid
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Goldfish
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Ion Channels / drug effects
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Ion Channels / physiology
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Membrane Potentials / drug effects
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Myelin Proteins / analysis
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Nerve Regeneration
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Neuroglia / cytology
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Neuroglia / drug effects
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Neuroglia / physiology*
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Oligodendroglia / cytology
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Oligodendroglia / physiology
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Optic Nerve / cytology
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Optic Nerve / physiology*
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Visual Pathways / cytology
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Visual Pathways / physiology*
Substances
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Glial Fibrillary Acidic Protein
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Glutamates
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Ion Channels
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Myelin Proteins
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Glutamic Acid
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4-Aminopyridine