Abstract
We assessed the influence that consecutive-day blood sampling, compared with single-day blood sampling, had on polymerase chain reaction (PCR)-adjusted parasitological cure after stepwise genotyping of merozoite surface proteins 2 (msp2) and 1 (msp1) in 106 children in Tanzania who had uncomplicated falciparum malaria treated with either sulfadoxine-pyrimethamine or artemether-lumefantrine; 78 of these children developed recurrent parasitemia during the 42-day follow-up period. Initial msp2 genotyping identified 27 and 33 recrudescences by use of single- and consecutive-day sampling, respectively; in subsequent msp1 genotyping, 17 and 21 of these episodes, respectively, were still classified as recrudescences; these results indicate a similar sensitivity of the standard single-day PCR protocol--that is, 82% (27/33) and 81% (17/21), in both genotyping steps. Interpretation of PCR-adjusted results will significantly depend on methodology.
Publication types
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Clinical Trial
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Protozoan / genetics
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Antimalarials / therapeutic use
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Artemether, Lumefantrine Drug Combination
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Artemisinins / therapeutic use*
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DNA, Protozoan / blood*
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DNA, Protozoan / genetics
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Drug Combinations
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Ethanolamines / therapeutic use*
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Fluorenes / therapeutic use*
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Genotype
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Humans
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Malaria, Falciparum / drug therapy*
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Malaria, Falciparum / parasitology*
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Merozoite Surface Protein 1 / genetics
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Plasmodium falciparum / classification
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Plasmodium falciparum / genetics
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Plasmodium falciparum / isolation & purification*
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Polymerase Chain Reaction*
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Protozoan Proteins / genetics
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Pyrimethamine / therapeutic use*
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Recurrence
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Sulfadoxine / therapeutic use*
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Tanzania
Substances
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Antigens, Protozoan
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Antimalarials
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Artemether, Lumefantrine Drug Combination
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Artemisinins
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DNA, Protozoan
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Drug Combinations
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Ethanolamines
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Fluorenes
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Merozoite Surface Protein 1
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Protozoan Proteins
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merozoite surface protein 2, Plasmodium
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fanasil, pyrimethamine drug combination
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Sulfadoxine
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Pyrimethamine