Abstract
This study focused on the stability of UCP2 (uncoupling protein 2), a mitochondrial carrier located in the inner membrane of mitochondrion. UCP2 is very unstable, with a half-life close to 30min, compared to 30h for its homologue UCP1, a difference that may highlight different physiological functions. Heat production by UCP1 in brown adipocytes is generally a long and adaptive phenomenon, whereas control of mitochondrial ROS by UCP2 needs more subtle regulation. We show that a mutation in UCP2 shown to modify its activity, actually decreases its stability.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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CHO Cells
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Cell Line
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Cricetinae
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Cricetulus
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DNA / genetics
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Drug Stability
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Half-Life
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Humans
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Ion Channels / genetics
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Ion Channels / metabolism*
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Mice
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Mice, Inbred C57BL
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Mitochondria / metabolism
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Mitochondrial Proteins / genetics
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Mitochondrial Proteins / metabolism*
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Mutagenesis, Site-Directed
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Uncoupling Protein 1
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Uncoupling Protein 2
Substances
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Ion Channels
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Mitochondrial Proteins
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Recombinant Proteins
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UCP1 protein, human
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UCP2 protein, human
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Ucp1 protein, mouse
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Ucp2 protein, mouse
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Uncoupling Protein 1
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Uncoupling Protein 2
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DNA