Comparison of mathematic models for assessment of glomerular filtration rate with electron-beam CT in pigs

Radiology. 2007 Feb;242(2):417-24. doi: 10.1148/radiol.2422052144.

Abstract

Purpose: To prospectively compare in pigs three mathematic models for assessment of glomerular filtration rate (GFR) on electron-beam (EB) computed tomographic (CT) images, with concurrent inulin clearance serving as the reference standard.

Materials and methods: This study was approved by the institutional animal care and use committee. Inulin clearance was measured in nine pigs (18 kidneys) and compared with single-kidney GFR assessed from renal time-attenuation curves (TACs) obtained with EB CT before and after infusion of the vasodilator acetylcholine. CT-derived GFR was calculated with the original and modified Patlak methods and with previously validated extended gamma variate modeling of first-pass cortical TACs. Statistical analysis was performed to assess correlation between CT methods and inulin clearance for estimation of GFR with least-squares regression analysis and Bland-Altman graphical representation. Comparisons within groups were performed with a paired t test.

Results: GFR assessed with the original Patlak method indicated poor correlation with inulin clearance, whereas GFR assessed with the modified Patlak method (P < .001, r = 0.75) and with gamma variate modeling (P < .001, r = 0.79) correlated significantly with inulin clearance and indicated an increase in response to acetylcholine.

Conclusion: CT-derived estimates of GFR can be significantly improved by modifications in image analysis methods (eg, use of a cortical region of interest).

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / pharmacology
  • Animals
  • Contrast Media
  • Glomerular Filtration Rate / physiology*
  • Image Processing, Computer-Assisted / methods
  • Inulin / pharmacokinetics
  • Iopamidol
  • Kidney / diagnostic imaging
  • Kidney / physiology*
  • Kidney Cortex / diagnostic imaging
  • Kidney Cortex / physiology
  • Kidney Function Tests
  • Kidney Medulla / diagnostic imaging
  • Kidney Medulla / physiology
  • Kidney Tubules / diagnostic imaging
  • Kidney Tubules / physiology
  • Metabolic Clearance Rate
  • Models, Biological*
  • Subtraction Technique
  • Swine
  • Tomography, X-Ray Computed / methods*
  • Vasodilator Agents / pharmacology

Substances

  • Contrast Media
  • Vasodilator Agents
  • Inulin
  • Iopamidol
  • Acetylcholine