Abstract
In the screening of antitumor compounds from microbial secondary metabolites, myxochelin A was isolated from a culture broth of Nonomuraea pusilla TP-A0861. The absolute configuration was determined to be S by synthesizing both enantiomers from an L- or D-lysine derivative and comparing their specific rotations. Both enantiomers of myxochelin A showed remarkable inhibitory effects on the invasion of murine colon 26-L5 carcinoma cells at non-cytotoxic concentrations.
MeSH terms
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Actinobacteria / chemistry*
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Actinobacteria / metabolism
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Actinobacteria / ultrastructure
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Animals
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Antineoplastic Agents / chemical synthesis
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Antineoplastic Agents / chemistry*
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Antineoplastic Agents / isolation & purification
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Antineoplastic Agents / pharmacology*
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Cell Adhesion / drug effects
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Cell Line, Tumor
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Cell Movement / drug effects
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Colonic Neoplasms / drug therapy
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Colonic Neoplasms / pathology
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Lysine / analogs & derivatives*
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Lysine / chemical synthesis
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Lysine / chemistry
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Lysine / isolation & purification
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Lysine / pharmacology
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Mice
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Microscopy, Electron, Scanning
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Nuclear Magnetic Resonance, Biomolecular
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Spectrometry, Mass, Fast Atom Bombardment
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Stereoisomerism
Substances
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Antineoplastic Agents
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myxochelin A
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Lysine