Abstract
Intracellular parasitism by Chlamydiales is a complex process involving transmission of metabolically inactive particles that differentiate, replicate, and re-differentiate within the host cell. A type three secretion system (T3SS) has been implicated in this process. We have here identified small molecules of a chemical class of acylated hydrazones of salicylaldehydes that specifically blocks the T3SS of Chlamydia. These compounds also affect the developmental cycle showing that the T3SS has a pivotal role in the pathogenesis of Chlamydia. Our results suggest a previously unexplored avenue for development of novel anti-chlamydial drugs.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Anti-Bacterial Agents / chemistry
-
Anti-Bacterial Agents / pharmacology*
-
Bacterial Proteins / antagonists & inhibitors*
-
Bacterial Proteins / genetics
-
Bacterial Proteins / metabolism*
-
Cell Proliferation / drug effects
-
Chlamydia Infections / microbiology*
-
Chlamydia trachomatis / cytology
-
Chlamydia trachomatis / drug effects
-
Chlamydophila pneumoniae / cytology
-
Chlamydophila pneumoniae / drug effects*
-
Dose-Response Relationship, Drug
-
Down-Regulation / drug effects
-
Epithelial Cells / cytology
-
Epithelial Cells / drug effects
-
Epithelial Cells / microbiology
-
Gene Expression Regulation, Bacterial / drug effects
-
Genes, Bacterial
-
HeLa Cells
-
Humans
-
Mice
-
Transcription, Genetic / drug effects
-
Yersinia Infections
-
Yersinia pseudotuberculosis / drug effects*
Substances
-
Anti-Bacterial Agents
-
Bacterial Proteins