Abstract
Objective:
To study the effect of adenovirus (Ad)-mediated fusion gene system driven by KDR promoter on the proliferation of human stomach adneocarcinoma SCG7901.
Methods:
The KDR-expressing SCG7901 cells and HepG2 cells that did not express KDR were both transfected with AdEasy-KDR-CDglyTK followed by treatment with the prodrugs 5-FC and/or GCV at different concentrations. The killing effects of the transfection on the cells were evaluated.
Results:
The expression of green fluorescent protein (GFP) was observed in 95% of the infected SCG7901 and HepG2 cells with the multiple of infection (MOI) of the Ads of 100. Transfection of SCG7901 and HepG2 cells did not produce significant changes in the cell growth, and the infected cells exhibited different sensitivities to the two prodrug: SCG7901 cells infected with rAd were highly sensitive to the prodrugs, but the infected HepG2 cells were not (P<0.01). The killing effect of CDglyTK fusion gene on the target cells was much stronger than that of either the single suicide gene (P<0.01).
Conclusion:
CDglyTK fusion gene system driven by KDR promoter selectively kills the KDR-CDglyTK SCG7901 cells and inhibits their proliferation.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / genetics
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Adenocarcinoma / metabolism
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Adenocarcinoma / pathology
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Adenoviridae / genetics*
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Cell Line, Tumor
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Cell Proliferation / drug effects*
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Cell Survival / drug effects
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Dose-Response Relationship, Drug
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Flucytosine / pharmacology*
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Ganciclovir / pharmacology*
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Genes, Transgenic, Suicide / genetics*
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Genetic Vectors
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Green Fluorescent Proteins / biosynthesis
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Green Fluorescent Proteins / genetics
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Humans
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Neovascularization, Pathologic / genetics
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Neovascularization, Pathologic / metabolism
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Neovascularization, Pathologic / pathology
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Prodrugs / pharmacology
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Stomach Neoplasms / genetics
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Stomach Neoplasms / metabolism
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Stomach Neoplasms / pathology
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Transfection
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Vascular Endothelial Growth Factor Receptor-2 / biosynthesis
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Vascular Endothelial Growth Factor Receptor-2 / genetics*
Substances
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Prodrugs
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RNA, Messenger
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Recombinant Fusion Proteins
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Green Fluorescent Proteins
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Flucytosine
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Vascular Endothelial Growth Factor Receptor-2
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Ganciclovir