Abstract
EXAFS analysis of Zn binding site(s) in bovine-heart cytochrome c oxidase and characterization of the inhibitory effect of internal zinc on respiratory activity and proton pumping of the liposome reconstituted oxidase are presented. EXAFS identifies tetrahedral coordination site(s) for Zn(2+) with two N-histidine imidazoles, one N-histidine imidazol or N-lysine and one O-COOH (glutamate or aspartate), possibly located at the entry site of the proton conducting D pathway in the oxidase and involved in inhibition of the oxygen reduction catalysis and proton pumping by internally trapped zinc.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Binding Sites / drug effects
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Cattle
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Cell Membrane / drug effects
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Cell Membrane / enzymology
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Electron Transport / drug effects
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Electron Transport Complex IV / antagonists & inhibitors*
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Electron Transport Complex IV / chemistry*
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Electron Transport Complex IV / metabolism
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Horses
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Liposomes
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Models, Molecular
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Protein Subunits / chemistry
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Protein Subunits / metabolism
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Proton Pumps / drug effects
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Protons
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Zinc / metabolism*
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Zinc / pharmacology*
Substances
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Liposomes
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Protein Subunits
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Proton Pumps
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Protons
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Electron Transport Complex IV
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Zinc