In this study, the effect of ultrasound exposure on number and scattering intensity from microbubbles in blood vessel model is noticed. Experimentally, microbubbles are produced using surfactant solution, and the measurement of number and scattering intensity of microbubbles are performed. Furthermore, theoretical calculation of scattering intensity from microbubbles in blood vessel model is performed to compare with experimental results. From the comparison of calculation and experiment, the effectiveness of constructed calculation model is confirmed.