Abstract
The flaviviral tick-borne encephalitis virus (TBEV) is a human pathogen having significant impact on public health. The geographical distribution of TBEV and TBEV-like viruses is increasing, which makes it important to characterise the natural virus populations. Here we present four RT-PCR strategies designed for detection of broad types of tick-borne flaviviruses. Sequence information on more than 32% of a TBEV genome was generated from a small pool of ticks collected in the Stockholm archipelago on the island of Torö. The sequences were characterised and compared with those of other tick-borne flaviviruses, which classified the virus as Western European TBEV.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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5' Untranslated Regions / genetics
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Animals
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Base Sequence
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DNA Primers
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Encephalitis Viruses, Tick-Borne / classification
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Encephalitis Viruses, Tick-Borne / genetics*
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Encephalitis Viruses, Tick-Borne / isolation & purification
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Environmental Monitoring
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Evolution, Molecular
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Ixodes / virology*
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Molecular Sequence Data
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Phylogeny
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RNA, Viral / genetics
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RNA, Viral / isolation & purification
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Sequence Alignment
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Species Specificity
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Sweden
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Viral Envelope Proteins / genetics
Substances
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5' Untranslated Regions
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DNA Primers
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RNA, Viral
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Viral Envelope Proteins