Abstract
This work aimed at developing a procedure for spore quantification. Spore content was determined by analyzing dipicolinic acid (dpa) extracted from aerobic granules by 13 methods. Concentrated HCl was able to release dpa completely. Results showed that dpa constituted 33.7 mg per g SS, meaning that about 337 mg per g SS were spores, not the normal vegetative cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteria, Aerobic / classification
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Bacteria, Aerobic / metabolism*
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Bioreactors
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Microscopy, Electron, Scanning
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Microscopy, Electron, Transmission
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Picolinic Acids / isolation & purification
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Picolinic Acids / metabolism*
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Spores, Bacterial / metabolism*
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Spores, Bacterial / ultrastructure
Substances
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Picolinic Acids
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dipicolinic acid