Abstract
Newcastle disease viruses isolated from Hong Kong live bird markets (LBMs) were not detected by a USDA-validated matrix gene real-time reverse transcription-PCR (RT-PCR) assay. Based upon phylogenetic analysis of the fusion gene, these viruses were related to lentogenic class I viruses found in U.S. LBMs and wild waterfowl. An alternative real-time RT-PCR assay which complements the matrix gene assay was developed to efficiently detect class I viruses.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Birds
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Hong Kong
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Molecular Sequence Data
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Newcastle Disease / virology*
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Newcastle disease virus / classification*
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Newcastle disease virus / genetics
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Newcastle disease virus / isolation & purification*
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Phylogeny
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RNA, Viral / analysis
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RNA, Viral / genetics
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Sequence Analysis, DNA
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Viral Fusion Proteins / genetics
Substances
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RNA, Viral
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Viral Fusion Proteins
Associated data
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GENBANK/EF027142
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GENBANK/EF027143
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GENBANK/EF027144
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GENBANK/EF027145
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GENBANK/EF027146
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GENBANK/EF027147
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GENBANK/EF027148
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GENBANK/EF027149
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GENBANK/EF027150
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GENBANK/EF027151
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GENBANK/EF027152
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GENBANK/EF027153
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GENBANK/EF027154
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GENBANK/EF027155
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GENBANK/EF027156
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GENBANK/EF027157
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GENBANK/EF027158
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GENBANK/EF027159
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GENBANK/EF027160
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GENBANK/EF027161
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GENBANK/EF027162
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GENBANK/EF027163
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GENBANK/EF027164