Abstract
The human gene Teratocarcinoma-derived growth factor 1 (TDGF1)/Cripto-1/CR-1 which is expressed in a wide variety of human carcinomas is a member of the EGF-cripto FRL1 cryptic (EGF-CFC) gene family. A majority of human colorectal tumors and hepatomas are known to possess a constitutively active canonical Wnt/beta-catenin/TCF signaling pathway, also express CR-1. Expression of a short form of CR-1 mRNA in colon carcinoma and hepatoma cell lines suggests that there may be differential regulation of CR-1 expression by the canonical Wnt signaling pathway in colon cancer as well as hepatoma cell lines. The present study demonstrates a direct transcriptional regulation of the short form CR-1 expression by the canonical Wnt signaling pathway through an intronic-exonic enhancer element, containing three tandem TCF/LEF binding sites within the CR-1 gene.
MeSH terms
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Binding Sites
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Carcinoma
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Carcinoma, Hepatocellular
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Cell Line, Tumor
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Colonic Neoplasms
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Enhancer Elements, Genetic
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Epidermal Growth Factor / genetics*
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GPI-Linked Proteins
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Gene Expression Regulation, Neoplastic*
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Genetic Vectors
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Humans
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Intercellular Signaling Peptides and Proteins
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Liver Neoplasms
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Lymphoid Enhancer-Binding Factor 1 / genetics
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Lymphoid Enhancer-Binding Factor 1 / metabolism*
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Membrane Glycoproteins / genetics*
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Neoplasm Proteins / genetics*
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Protein Isoforms / genetics
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RNA, Messenger / genetics
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Recombinant Proteins / metabolism
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TCF Transcription Factors / genetics
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TCF Transcription Factors / metabolism*
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Transcription, Genetic
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beta Catenin / metabolism*
Substances
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GPI-Linked Proteins
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Intercellular Signaling Peptides and Proteins
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Lymphoid Enhancer-Binding Factor 1
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Membrane Glycoproteins
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Neoplasm Proteins
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Protein Isoforms
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RNA, Messenger
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Recombinant Proteins
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TCF Transcription Factors
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TDGF1 protein, human
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beta Catenin
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Epidermal Growth Factor