Abstract
Cytokines control cell differentiation, proliferation, and function by regulating gene expression program. Physiological roles and induction mechanisms of cytokine-inducible genes are not fully understood. Here, we identified a novel immediate-early cytokine-responsive gene, cyclon (cytokine-induced protein with coiled-coil domain), which is induced in a hematopoietic cell line by interleukin 3 (IL-3). cyclon gene encodes a phosphorylated nuclear protein consisting of repetitive sequences in the amino-terminus and a coiled-coil domain in the carboxyl-terminus. A novel transient reporter assay revealed that mouse cyclon promoter contains redundant elements for IL-3-induced gene expression.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Line
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Cloning, Molecular
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DNA Primers / genetics
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Gene Expression / drug effects
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Humans
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Interleukin-3 / pharmacology*
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Mice
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Molecular Sequence Data
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Molecular Weight
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Oligonucleotide Array Sequence Analysis
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Phosphorylation
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Promoter Regions, Genetic*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Sequence Homology, Amino Acid
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Suppressor of Cytokine Signaling Proteins / chemistry
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Suppressor of Cytokine Signaling Proteins / genetics*
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Suppressor of Cytokine Signaling Proteins / metabolism
Substances
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DNA Primers
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Interleukin-3
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RNA, Messenger
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Suppressor of Cytokine Signaling Proteins
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cytokine inducible SH2-containing protein
Associated data
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GENBANK/DQ501251
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GENBANK/DQ501252
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GENBANK/DQ501253