Abstract
Notch signalling affects most aspects of development, not least the determination of neural stem cell fate. Here, we describe the presence of the Notch-1 intracellular domain (N1(ICD)) in sub-nuclear bodies in SH-SY5Y neuroblastomas and in primary rat cortical neurons as well as several other mammalian cell lines. We also demonstrate that these N1(ICD)-positive sub-nuclear bodies are distinct from premyelocytic leukaemia (PML) and SC35 bodies. Furthermore, using Notch deletion constructs we determined that a region C-terminal of amino acid 2094 is involved in targeting the N1(ICD) into sub-nuclear bodies. These findings have ramifications for nuclear architecture and gene transcription.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Nucleus / metabolism*
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Cells, Cultured
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Cerebral Cortex / cytology*
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Cricetinae
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Cricetulus
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Embryo, Mammalian
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Green Fluorescent Proteins / metabolism
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Humans
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Mutagenesis / physiology
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Neoplasm Proteins / metabolism
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Neuroblastoma / pathology*
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Neurons / metabolism*
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Neurons / ultrastructure
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Nuclear Proteins / metabolism
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Promyelocytic Leukemia Protein
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Protein Structure, Tertiary / physiology
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Rats
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Receptor, Notch1 / metabolism*
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Transcription Factors / metabolism
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Transfection / methods
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Tumor Suppressor Proteins / metabolism
Substances
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NOTCH1 protein, human
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Neoplasm Proteins
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Nuclear Proteins
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Promyelocytic Leukemia Protein
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Receptor, Notch1
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Transcription Factors
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Tumor Suppressor Proteins
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PML protein, human
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Green Fluorescent Proteins