Tanshinone IIA from Salvia miltiorrhiza induces heme oxygenase-1 expression and inhibits lipopolysaccharide-induced nitric oxide expression in RAW 264.7 cells

Mitochondrion. 2007 Feb-Apr;7(1-2):101-5. doi: 10.1016/j.mito.2006.11.018. Epub 2006 Dec 9.

Abstract

Tanshinone IIA exerts anti-inflammatory effects and influences electron transfer reaction in mitochondria. In the present study, we demonstrated that tanshinone IIA increased intracellular production of reactive oxygen species (ROS), which in turn induces heme oxygenase-1 (HO-1) expression in RAW 264.7 macrophages. Tanshinone IIA inhibited COX-2 and iNOS expression in lipopolysaccharide-activated RAW 264.7 macrophages. Inhibition of HO-1 or scavenging of CO significantly reversed the inhibition of LPS-stimulated nitrite accumulation by tanshinone IIA, suggesting a novel role of HO-1 in the anti-inflammatory effect of tanshinone IIA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abietanes
  • Animals
  • Cell Line
  • Cyclooxygenase 2 / biosynthesis
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Heme Oxygenase-1 / biosynthesis*
  • Lipopolysaccharides / pharmacology
  • Macrophages / enzymology
  • Membrane Proteins / biosynthesis*
  • Mice
  • Nitric Oxide Synthase Type II / antagonists & inhibitors
  • Phenanthrenes / pharmacology*
  • Salvia miltiorrhiza / chemistry
  • Signal Transduction

Substances

  • Abietanes
  • Lipopolysaccharides
  • Membrane Proteins
  • Phenanthrenes
  • tanshinone
  • Nitric Oxide Synthase Type II
  • Nos2 protein, mouse
  • Heme Oxygenase-1
  • Hmox1 protein, mouse
  • Cyclooxygenase 2
  • Extracellular Signal-Regulated MAP Kinases