Abstract
We observed vitamin B(12)-mediated growth inhibition of Mycobacterium tuberculosis strain CDC1551. The B(12) sensitivity was mapped to a polymorphism in metH, encoding a coenzyme B(12)-dependent methionine synthase. Vitamin B(12)-resistant suppressor mutants of CDC1551 containing mutations in a B(12) riboswitch upstream of the metE gene, which encodes a B(12)-independent methionine synthase, were isolated. Expression analysis confirmed that the B(12) riboswitch is a transcriptional regulator of metE in M. tuberculosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / biosynthesis*
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5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / genetics
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / genetics
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Cobamides / pharmacology
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Gene Expression Regulation, Bacterial*
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Growth Inhibitors / pharmacology
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Molecular Structure
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Mycobacterium tuberculosis / genetics
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Mycobacterium tuberculosis / growth & development
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Mycobacterium tuberculosis / physiology*
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Nucleic Acid Conformation
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RNA, Bacterial / biosynthesis
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RNA, Messenger / biosynthesis
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Suppression, Genetic
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Transcription, Genetic
Substances
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Bacterial Proteins
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Cobamides
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Growth Inhibitors
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RNA, Bacterial
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RNA, Messenger
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5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase