Direct interaction between two Escherichia coli transcription antitermination factors, NusB and ribosomal protein S10

S W Mason; J Li; J Greenblatt

doi:10.1016/0022-2836(92)90715-v

Direct interaction between two Escherichia coli transcription antitermination factors, NusB and ribosomal protein S10

J Mol Biol. 1992 Jan 5;223(1):55-66. doi: 10.1016/0022-2836(92)90715-v.

Authors

S W Mason¹, J Li, J Greenblatt

Affiliation

¹ Department of Molecular and Medical Genetics, University of Toronto, Canada.

PMID: 1731086
DOI: 10.1016/0022-2836(92)90715-v

Abstract

The Escherichia coli proteins NusB and ribosomal protein S10 are important for transcription antitermination by the bacteriophage lambda N protein. We have used sucrose gradient co-sedimentation and affinity chromatography with immobilized ribosomal protein S10, a glutathione S-transferase-S10 fusion protein, and NusB to show that NusB binds directly and very selectively to S10. The interaction is non-ionic and has an estimated Kd value of 10(-7) M. We hypothesize that NusB binds to N-modified transcription complexes primarily by interacting with S10.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics*
Chromatography, Affinity
Escherichia coli / genetics*
Escherichia coli Proteins*
Gene Expression Regulation, Bacterial*
Mutation
Protein Binding
Recombinant Fusion Proteins / metabolism
Ribosomal Proteins / genetics*
Terminator Regions, Genetic*
Transcription Factors
Transcription, Genetic*

Substances

Bacterial Proteins
Escherichia coli Proteins
NusB protein, E coli
Recombinant Fusion Proteins
Ribosomal Proteins
Transcription Factors
ribosomal protein S10