Abstract
The discriminatory potential of a combination of various typing methods was evaluated on a set of 21 Clostridium difficile isolates obtained from symptomatic patients hospitalized in a geriatric unit and 7 non-toxigenic isolates from the same hospital. Isolates were firstly serotyped and toxinotyped. Of the 28 isolates, 19 belonged to serogroup A. PCR-ribotyping and PCR-RFLP on the fliC and slpA genes were then applied to these 19 isolates. The results suggest that the combination of PCR-ribotyping with PCR-RFLP analysis of slpA could be more discriminatory and suitable for studying C. difficile epidemiology.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Toxins / analysis
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Bacterial Typing Techniques / methods*
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Clostridioides difficile / classification*
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Clostridioides difficile / genetics*
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Clostridioides difficile / isolation & purification
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Cross Infection / microbiology*
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Enterocolitis, Pseudomembranous / microbiology*
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France
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Genotype
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Geriatrics
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Hospitals, Special*
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Humans
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Molecular Epidemiology / methods
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Phenotype
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Polymorphism, Restriction Fragment Length
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Ribotyping
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Serotyping
Substances
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Bacterial Proteins
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Bacterial Toxins
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surface layer protein A, Bacteria