Comparison between synthetic nuclear localization signal peptides from the steroid/thyroid hormone receptors superfamily

Biochem Biophys Res Commun. 1992 Jan 15;182(1):289-93. doi: 10.1016/s0006-291x(05)80143-3.

Abstract

The main objective of the study is to demonstrate that short basic peptides from the steroid/thyroid hormone receptors superfamily act as Nuclear Localization Signals out of receptors context. Such synthesized peptides, chemically coupled to Bovine Serum Albumin, were shown to enable the corresponding BSA-conjugate to be transported to the nucleus. A second objective is to demonstrate the utility of viral cointernalization as a good method for rapid quantitation, comparison and competition in nuclear entry.

Publication types

  • Comparative Study

MeSH terms

  • Adenoviridae / genetics
  • Amino Acid Sequence
  • Carrier Proteins / metabolism
  • Cell Nucleus / physiology*
  • Humans
  • KB Cells
  • Kinetics
  • Molecular Sequence Data
  • Multigene Family*
  • Oligopeptides / chemical synthesis
  • Protein Sorting Signals / genetics*
  • Receptors, Steroid / genetics*
  • Receptors, Thyroid Hormone / genetics*
  • Sequence Homology, Nucleic Acid

Substances

  • Carrier Proteins
  • Oligopeptides
  • Protein Sorting Signals
  • Receptors, Steroid
  • Receptors, Thyroid Hormone