Synthesis of two carboxylic haptens for raising antibodies to 25-hydroxyvitamin D3 and 1alpha,25-dihydroxyvitamin D3

Carlos Gregorio; Silvina Eduardo; Luis C Rodrigues; María A Regueira; Ramón Fraga; Ricardo Riveiros; Miguel Maestro; Antonio Mouriño

doi:10.1016/j.jsbmb.2007.01.001

Synthesis of two carboxylic haptens for raising antibodies to 25-hydroxyvitamin D3 and 1alpha,25-dihydroxyvitamin D3

J Steroid Biochem Mol Biol. 2007 Mar;103(3-5):227-30. doi: 10.1016/j.jsbmb.2007.01.001. Epub 2007 Jan 18.

Authors

Carlos Gregorio¹, Silvina Eduardo, Luis C Rodrigues, María A Regueira, Ramón Fraga, Ricardo Riveiros, Miguel Maestro, Antonio Mouriño

Affiliation

¹ Departamento de Química Orgánica y Unidad Asociada al C.S.I.C., Universidad de Santiago de Compostela, E-15706 Santiago de Compostela, Spain.

PMID: 17321129
DOI: 10.1016/j.jsbmb.2007.01.001

Abstract

Hapten derivatives of 25-hydroxyvitamin D(3) and 1alpha,25-dihydroxyvitamin D(3) were synthesized using the Wittig-Horner approach. Both haptens bearing a carboxylic group at the side chain that can be linked to a protein for raising antibodies of potential utility for the determination of 25-hydroxyvitamin D(3), 1alpha,25-dihydroxyvitamin D(3) and 1alpha-hydroxylated vitamin D(3) analogues.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Calcifediol / chemical synthesis*
Calcifediol / chemistry
Calcifediol / immunology
Calcifediol / metabolism
Haptens / chemistry*
Haptens / immunology
Molecular Structure
Vitamin D / analogs & derivatives*
Vitamin D / chemical synthesis
Vitamin D / chemistry
Vitamin D / immunology
Vitamin D / metabolism

Substances

Haptens
dihydroxy-vitamin D3
Vitamin D
Calcifediol