Abstract
A new NADH-fumarate reductase inhibitor, verticipyrone, was isolated from the cultured broth of a fungus, Verticillium sp. FKI-1083. The structure was established as (E)-2-methoxy-3,5-dimethyl-6-(3-methyl-2-undecenyl)-4H-pyran-4-one. Verticipyrone exhibited an IC50 value of 0.88 nM against NADH-fumarate reductase of Ascaris suum. Verticipyrone inhibited both Ascaris and bovine heart complex I, and its synthetic analogue, 8,9-dihydro-8-hydroxyverticipyrone, showed good selectivity against Ascaris complex I.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkenes / chemistry
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Alkenes / isolation & purification*
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Alkenes / pharmacology
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Enzyme Inhibitors / chemistry
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Enzyme Inhibitors / isolation & purification*
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Enzyme Inhibitors / pharmacology
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Fermentation
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Magnetic Resonance Spectroscopy
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Oxidoreductases Acting on CH-CH Group Donors / antagonists & inhibitors*
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Pyrones / chemistry
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Pyrones / isolation & purification*
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Pyrones / pharmacology
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Verticillium / metabolism*
Substances
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Alkenes
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Enzyme Inhibitors
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Pyrones
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verticipyrone
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Oxidoreductases Acting on CH-CH Group Donors
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fumarate reductase (NADH)