Abstract
Mito-DEPMPO, a new DEPMPO analogue bearing a triphenylphosphonium group, was synthesized via a novel NH2-reactive DEPMPO. The half-life of the Mito-DEPMPO superoxide adduct was estimated to be ca. 40 min. Using Mito-DEPMPO, reactive oxygen species generated in intact mitochondria were detected and characterized by EPR.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cyclic N-Oxides / chemical synthesis*
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Cyclic N-Oxides / chemistry
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Electron Spin Resonance Spectroscopy
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Hypoxanthine / metabolism
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Mice
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Mitochondria / metabolism*
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Osteoclasts / cytology
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Osteoclasts / metabolism
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Spin Labels*
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Spin Trapping
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Superoxide Dismutase / metabolism
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Superoxides / metabolism*
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Xanthine Oxidase / metabolism
Substances
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Cyclic N-Oxides
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Spin Labels
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Superoxides
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5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide
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Hypoxanthine
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Superoxide Dismutase
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Xanthine Oxidase