Abstract
We have identified a serum-inducible gene, relB, which encodes a protein of 558 amino acids containing a region with high similarity to c-Rel and other members of the Rel family. Transcriptional activation analysis of GAL4-RelB fusion proteins in yeast cells reveals that RelB contains in its C-terminal 180 amino acids a transcriptional activation domain. The N-terminal part including the region of similarity with the Rel family shows no detectable transcriptional activity. RelB does not bind with high affinity to NF-kappa B sites, but heterodimers between RelB and p50-NF-kappa B do bind to different NF-kappa B-binding sites with a similar affinity to that shown by p50-NF-kappa B homodimers. However, RelB/p50-NF-kappa B heterodimers, in contrast to p50-NF-kappa B homodimers, transactivate transcription of a promoter containing a kappa B-binding site.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Amino Acid Sequence
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Animals
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Base Sequence
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DNA-Binding Proteins
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Gene Expression Regulation / physiology
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Mice
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Molecular Sequence Data
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Multigene Family / genetics
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NF-kappa B / metabolism*
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Oncogene Proteins v-rel
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Oncogenes / genetics*
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Precipitin Tests
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Retroviridae Proteins, Oncogenic / chemistry
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Retroviridae Proteins, Oncogenic / genetics
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Retroviridae Proteins, Oncogenic / metabolism*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Transcription Factors / metabolism*
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beta-Galactosidase / genetics
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beta-Galactosidase / metabolism
Substances
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DNA-Binding Proteins
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Fungal Proteins
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GAL4 protein, S cerevisiae
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NF-kappa B
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Oncogene Proteins v-rel
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Recombinant Fusion Proteins
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Retroviridae Proteins, Oncogenic
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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beta-Galactosidase