Abstract
Nanoparticles in biological fluids almost invariably become coated with proteins that may confer nanomedical and nanotoxicological effects. Understanding these effects requires quantitative measurements using simple systems. Adsorption of HSA to copolymer nanoparticles of varying hydrophobicity and curvature was studied using ITC, yielding stoichiometry, affinity, and enthalpy changes upon binding. The hydrophobicity was controlled via the co-monomer ratio, N-iso-propylacrylamide/N-tert-butylacrylamide. The most hydrophobic particles become fully covered with a single layer of protein, except at high curvature.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acrylamides / chemistry*
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Adsorption
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Coated Materials, Biocompatible / chemistry
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Computer Simulation
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Crystallization / methods
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Humans
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Hydrophobic and Hydrophilic Interactions
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Materials Testing
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Models, Chemical*
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Molecular Conformation
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Nanoparticles / chemistry*
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Nanoparticles / ultrastructure*
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Nanotechnology / methods*
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Particle Size
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Protein Binding
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Serum Albumin / chemistry*
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Serum Albumin / ultrastructure
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Surface Properties
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Thermodynamics
Substances
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Acrylamides
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Coated Materials, Biocompatible
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Serum Albumin
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N-isopropylacrylamide
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tert-butylacrylamide