Abstract
Mycothiol (MSH) [1-D-myo-inosityl-2-(N-acetyl-l-cysteinyl)amido-2-deoxy-alpha-D-glucopyranoside], isolated as the bimane derivative, was established to be the major thiol in Nocardia sp. strain NRRL 5646, a species most closely related to Nocardia brasiliensis strain DSM 43758(T). Thiol formation and detection of MSH-dependent formaldehyde dehydrogenase activity in cell extracts are relevant to the possible modulation of nitric oxide toxicity generated by strain NRRL 5646.
MeSH terms
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Aldehyde Oxidoreductases / metabolism
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Base Sequence
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Chromatography, High Pressure Liquid
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Cysteine / analysis
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Cysteine / isolation & purification*
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Glycopeptides / analysis
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Glycopeptides / isolation & purification*
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Inositol / analysis
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Inositol / isolation & purification*
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Molecular Sequence Data
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Molecular Structure
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Nitric Oxide / metabolism
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Nocardia / chemistry*
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Nocardia / genetics
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
Substances
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Glycopeptides
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RNA, Ribosomal, 16S
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mycothiol
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Nitric Oxide
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Inositol
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Aldehyde Oxidoreductases
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formaldehyde dehydrogenase, glutathione-independent
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Cysteine