Abstract
We have reported recently that intranasal (i.n.) vaccination with chlamydial protease-like activity factor (CPAF) and interleukin-12 (IL-12) enhances protective immunity against genital chlamydial challenge. In this study, we show that i.n. or intraperitoneal (i.p.) vaccination with CPAF plus CpG deoxynucleotides (CpG), an alternative T helper 1 (Th1) adjuvant, induced robust CPAF-specific IFN-gamma responses and elevated levels of serum antibody and vaginal IgA production. CPAF+CpG vaccinated animals displayed accelerated genital chlamydial clearance, and minimal hydrosalpinx and inflammatory cellular infiltration compared to mock-immunized (PBS) challenged animals. Together, CpG dexoynucleotides are an efficacious alternative Th1 adjuvant with CPAF to induce protective anti-chlamydial immunity.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Adjuvants, Immunologic / administration & dosage*
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Administration, Intranasal*
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Animals
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Antibodies, Bacterial / blood
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Bacterial Vaccines / administration & dosage
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Bacterial Vaccines / immunology
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Chlamydia Infections / immunology
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Chlamydia Infections / microbiology
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Chlamydia muridarum / immunology*
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CpG Islands / immunology
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Endopeptidases / administration & dosage*
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Female
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Genital Diseases, Female / immunology*
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Genital Diseases, Female / microbiology
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Humans
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Immunization
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Immunoglobulin A, Secretory / metabolism
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Interferon-gamma / metabolism
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Mice
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Mice, Inbred BALB C
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Oligodeoxyribonucleotides / administration & dosage*
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Vagina / immunology
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Vagina / microbiology
Substances
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Adjuvants, Immunologic
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Antibodies, Bacterial
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Bacterial Vaccines
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CPG-oligonucleotide
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Immunoglobulin A, Secretory
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Oligodeoxyribonucleotides
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Interferon-gamma
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Endopeptidases
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CPA factor