Assessment of the solid-state composition of an active salicylanilide compound by FT-Raman spectroscopy

J Pharm Biomed Anal. 2007 May 9;44(1):254-7. doi: 10.1016/j.jpba.2007.01.034. Epub 2007 Jan 30.

Abstract

A biologically active salicylanilide compound currently appears in three known solid-state forms: polymorph I (Pol I), polymorph II (Pol II) and the amorphous form (Amorph). The obtained FT-Raman spectra revealed several regions of interest (ROIs) qualitatively distinguishing the different forms, allowing samples with an unknown polymorphic composition to be quantitatively analysed by FT-Raman spectroscopy. The Markov-transformed peak areas of the Raman-bands in the ROIs from the samples were determined and compared with the transformed peak areas obtained for the reference solid-state forms. A constrainted linear regression model estimated the contribution of each reference to the different samples. The applicability of this approach was demonstrated by analysing commercially available batches.

MeSH terms

  • Markov Chains
  • Pharmaceutical Preparations / analysis*
  • Pharmaceutical Preparations / chemistry*
  • Reference Values
  • Salicylanilides / analysis*
  • Spectroscopy, Fourier Transform Infrared / methods*
  • Spectrum Analysis, Raman / methods*

Substances

  • Pharmaceutical Preparations
  • Salicylanilides
  • salicylanilide