Effect of the administration of fermentable and non-fermentable dietary fibre on intestinal bacterial translocation in ascitic cirrhotic rats

Clin Nutr. 2007 Jun;26(3):383-7. doi: 10.1016/j.clnu.2007.01.008. Epub 2007 Mar 26.

Abstract

Background: Bacterial infections are frequent in cirrhosis. Experimental studies suggest a pathogenic role of intestinal bacterial translocation in them. Both fermentable and non-fermentable fibre avoided intestinal bacterial translocation (IBT) in animal models of gut starvation and critical illness.

Aim: To assess the effect of fermentable (pectin) or non-fermentable (lignin) fibre on IBT in ascitic cirrhotic rats.

Methods: Thirty-six rats induced to cirrhosis with oral CCl4 were randomized (6 weeks after the first CCl4 dose) to receive rat chow+5% lignin (LIG, n=13), rat chow+5% pectin (PEC, n=13), or rat chow only (CON, n=10). Once ascites developed, animals were laparotomized and samples of mesenteric lymph nodes (MLN), ascitic fluid, portal and peripheral blood and liver, were obtained for culture.

Results: IBT rate was: LIG=5/13, PEC=4/13, CON=5/10 (P=N.S.). The median amount of translocated bacteria in rats with IBT was lower in the PEC group (2 x 10(2) CFU/g MLN), than in LIG (10(5) CFU/g MLN) and CON (10(4) CFU/g MLN) groups (P<0.05). All other samples were sterile except for a portal blood sample (Enterococcus faecalis) of the LIG group.

Conclusions: IBT incidence is not decreased by either pectin or lignin in ascitic cirrhotic rats, but pectin supplementation reduces the amount of translocated bacteria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ascites / microbiology*
  • Bacterial Translocation / drug effects*
  • Carbon Tetrachloride Poisoning / complications
  • Dietary Fiber / metabolism
  • Dietary Fiber / pharmacology*
  • Fermentation
  • Humans
  • Lignin / metabolism
  • Lignin / pharmacology
  • Liver Cirrhosis, Experimental / chemically induced
  • Liver Cirrhosis, Experimental / microbiology*
  • Male
  • Pectins / metabolism
  • Pectins / pharmacology
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Dietary Fiber
  • Pectins
  • Lignin