Multiplex PCR assay for rapid and accurate capsular typing of group B streptococci

J Clin Microbiol. 2007 Jun;45(6):1985-8. doi: 10.1128/JCM.00159-07. Epub 2007 Mar 21.

Abstract

We developed a simple, specific, and sensitive two-multiplex-PCR assay that enabled the detection of all known group B streptococcal (GBS) capsular polysaccharides. This test is well adapted for GBS capsular polysaccharide typing in large-scale epidemiological studies.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Capsules / chemistry
  • Bacterial Capsules / classification*
  • Bacterial Capsules / genetics
  • Bacterial Typing Techniques
  • Base Sequence
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Sequence Analysis, DNA
  • Streptococcal Infections / epidemiology*
  • Streptococcal Infections / microbiology
  • Streptococcus agalactiae / classification*
  • Streptococcus agalactiae / genetics
  • Time Factors

Associated data

  • GENBANK/AAJO01000077
  • GENBANK/AM498295
  • GENBANK/AM498296