Purpose: To investigate the effect of oculotect gel and alteration of goblet cells of rat after corneal transplantation.
Methods: 48 SD rats were randomly divided into oculotect gel-treated, 0.1% dexamethasone eye drops-treated, control groups and normal groups. After the rat model of heterograft-heterotopic graft rejection was made, slit-limp microscope and PAS stainings, light microscope and HE PAS staining technique were used to observe the number and morphological difference of goblet cells in conjunctiva between operative groups and normal groups.
Results: The HE PAS staining detection showed that the number of conjunctiva goblet cells in oculotect gel group 0.1% dexamethasone eye drops group and control groups is lower than that in normal group after surgery (P < 0.01). The number of conjunctiva goblet cells in oculotect gel group and 0.1% dexamethasone eye drops group is higher than that in control group (P < 0.05). That number in 0.1% dexamethasone eye drops group is higher than that in oculotect gel group (P < 0.05).
Conclusions: The results indicate that oculotect gel significantly inhibit the decrease of goblet cells after corneal allograft rejection in rats.