Abstract
Increased monoamine oxidase (MAO) activity was recently shown to accompany apoptotic cell death of various neuronal cells following growth factor deprivation. Here we show that in serum deprived SH-SY5Y cells, MAO-A mRNA levels and catalytic activities are increased, linked with activation of the apoptotic executioner caspase-3. Importantly, specific inhibition of MAO-A activity resulted in loss of apoptotic cell morphology. Our study indicates that MAO catalytic activity is involved in apoptotic signalling in response to serum withdrawal in neuronal cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / drug effects
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Apoptosis / physiology*
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Caspase 3 / metabolism
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Cell Line, Tumor
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Culture Media, Serum-Free / pharmacology
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Enzyme Activation / drug effects
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Enzyme Activation / physiology
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Humans
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Monoamine Oxidase / genetics
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Monoamine Oxidase / metabolism*
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Monoamine Oxidase Inhibitors / pharmacology
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Nerve Degeneration / chemically induced
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Nerve Degeneration / enzymology*
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Nerve Degeneration / physiopathology
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Neuroblastoma
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Neurons / drug effects
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Neurons / enzymology*
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RNA, Messenger / metabolism
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Up-Regulation / drug effects
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Up-Regulation / physiology
Substances
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Culture Media, Serum-Free
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Monoamine Oxidase Inhibitors
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RNA, Messenger
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Monoamine Oxidase
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Caspase 3