Genotype differentiating RT-PCR and sandwich ELISA: handy tools in epidemiological investigation of foot and mouth disease

J Virol Methods. 2007 Jul;143(1):117-21. doi: 10.1016/j.jviromet.2007.02.008. Epub 2007 Apr 2.

Abstract

Years of molecular epidemiological surveillance has revealed co-circulation of two antigenically divergent genotypes of foot and mouth disease virus serotype A in India. Genotype differentiating RT-PCR and sandwich ELISA were developed as fast, cost-effective and user-friendly alternatives to 1D region based phylogeny for detection and differentiation of genotype VI and VII. The RT-PCR assay targeting 1D region was found to be more sensitive and authentic in distinguishing genotypes than sandwich ELISA. These assays promise to be reliable tools in the epidemiological investigation of foot and mouth disease in the country.

MeSH terms

  • Animals
  • Antigens, Viral
  • Cattle
  • Cattle Diseases / diagnosis*
  • Cattle Diseases / immunology
  • Cattle Diseases / virology
  • Enzyme-Linked Immunosorbent Assay*
  • Foot-and-Mouth Disease / diagnosis*
  • Foot-and-Mouth Disease / epidemiology
  • Foot-and-Mouth Disease / immunology
  • Foot-and-Mouth Disease / virology
  • Foot-and-Mouth Disease Virus / classification
  • Foot-and-Mouth Disease Virus / immunology
  • Foot-and-Mouth Disease Virus / isolation & purification*
  • Genotype
  • India
  • Phylogeny
  • Reverse Transcriptase Polymerase Chain Reaction*
  • Serotyping

Substances

  • Antigens, Viral