Non-invasive measurement of bioelectric currents with a vibrating probe

Nat Protoc. 2007;2(3):661-9. doi: 10.1038/nprot.2007.91.

Abstract

Small d.c. electrical signals have been detected in many biological systems and often serve important functions in cells and organs. For example, we have recently found that they play a far more important role in directing cell migration in wound healing than previously thought. Here, we describe the manufacture and use of a simplified ultrasensitive vibrating probe system for measuring extracellular electrical currents. This vibrating probe is an insulated, sharpened metal wire with a small platinum-black tip (10-30 microm), which can detect ionic currents in the microA cm(-2) range in physiological saline. The probe is vibrated at about 300 Hz by a piezoelectric bender. In the presence of an ionic current, the probe detects a voltage difference between the extremes of its movement. The basic, low-cost system we describe is readily adaptable to most laboratories interested in measuring physiological electric currents associated with wounds, developing embryos and other biological systems.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Electrophysiology / instrumentation*
  • Electrophysiology / methods*
  • Extracellular Space / physiology*
  • Ion Channels / physiology*
  • Microelectrodes
  • Vibration

Substances

  • Ion Channels