Abstract
One home-developed assay and two commercial assays for the rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) were compared by use of a collection of clinical isolates displaying highly diverse genetic backgrounds. Our results suggest that users of orfX-staphylococcal cassette chromosome mec-based assays should repeatedly monitor the local epidemiology to minimize the risks of detection bias and the omission of emerging MRSA clones.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Typing Techniques
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DNA, Bacterial / analysis
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DNA, Bacterial / isolation & purification
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Humans
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Methicillin Resistance*
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Nucleic Acid Hybridization / methods*
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Penicillin-Binding Proteins
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Polymerase Chain Reaction / methods*
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Reagent Kits, Diagnostic*
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Staphylococcal Infections / diagnosis
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Staphylococcal Infections / epidemiology
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Staphylococcal Infections / microbiology*
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Staphylococcus aureus / classification*
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / genetics
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Time Factors
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Transcription Factors / genetics*
Substances
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Bacterial Proteins
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DNA, Bacterial
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Penicillin-Binding Proteins
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Reagent Kits, Diagnostic
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SarH1 protein, Staphylococcus aureus
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Transcription Factors
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mecA protein, Staphylococcus aureus