[Expression, purification, and characterization of an anti-human RBC ScFv-HIV gp160 fusion protein for hemagglutination-based rapid detection of antibodies to HIV in whole blood]

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2007 Mar;21(1):76-8.
[Article in Chinese]

Abstract

Objective: To construct and express anti-human RBC and HIVgp160 fusion protein for rapid detection of antibody to HIV.

Methods: The gene of the anti human RBC ScFv and HIV antigen were constructed together into expression vector. The fusion protein was expressed in E. coli.

Results: The fusion protein was proved to be able to bind both anti-RBC and HIVgp160. It could cause agglutination of human RBC when HIVgp160 was present.

Conclusion: The fusion protein has the potential in rapid detection of HIV.

Publication types

  • English Abstract

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / isolation & purification
  • Autoantibodies / immunology
  • Autoantibodies / isolation & purification
  • Cloning, Molecular
  • Enzyme-Linked Immunosorbent Assay
  • Erythrocytes / immunology*
  • Gene Expression
  • Genetic Vectors / genetics
  • HIV Antibodies / blood*
  • HIV Antibodies / immunology
  • HIV Envelope Protein gp160 / genetics
  • HIV Envelope Protein gp160 / immunology*
  • HIV Envelope Protein gp160 / metabolism
  • HIV Seropositivity / blood
  • Hemagglutination Tests / methods
  • Humans
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology*
  • Recombinant Fusion Proteins / metabolism

Substances

  • Antibodies, Monoclonal
  • Autoantibodies
  • HIV Antibodies
  • HIV Envelope Protein gp160
  • Recombinant Fusion Proteins