Abstract
Using a modified random arbitrarily primed PCR approach, the operon encoding the Enterococcus faecalis JH2-2 CroRS two-component regulatory system was shown to be repressed during stationary phase, and a CroRS-regulated operon (glnQHMP) was identified. Gel retardation assays showed that the CroR regulator binds specifically to the glnQHMP promoter.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / metabolism*
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Carrier Proteins / genetics
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DNA, Bacterial / metabolism
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DNA-Binding Proteins / metabolism
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Electrophoretic Mobility Shift Assay
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Enterococcus faecalis / genetics*
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Gene Expression Regulation, Bacterial*
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Operon / genetics
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Promoter Regions, Genetic
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Protein Binding
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Random Amplified Polymorphic DNA Technique / methods*
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Signal Transduction / genetics
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Transcription Factors / metabolism*
Substances
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Bacterial Proteins
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Carrier Proteins
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DNA, Bacterial
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DNA-Binding Proteins
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Transcription Factors
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glutamine transport proteins