Abstract
The approximately 14 kb mRNA of the polycystic kidney disease gene PKD1 encodes a large ( approximately 460 kDa) protein, termed polycystin-1 (PC-1), that is responsible for autosomal dominant polycystic kidney disease (ADPKD). The unique organization of its multiple adhesive domains (16 Ig-like domains/PKD domains) suggests that it may play an important role in cell-cell/cell-matrix interactions. Here we demonstrated that PKD1 promoted cell-cell and cell-matrix interactions in cancer cells, indicating that PC-1 is involved in the cell adhesion process. Furthermore in this study, we showed that PKD1 inhibited cancer cells migration and invasion. And we also showed that PC-1 regulated these processes in a process that may be at least partially through the Wnt pathway. Collectively, our data suggest that PKD1 may act as a novel member of the tumor suppressor family of genes.
Copyright (c) 2007 John Wiley & Sons, Ltd.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Blotting, Western
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Cadherins / metabolism
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Cell Adhesion / genetics
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Cell Adhesion / physiology
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Cell Aggregation / genetics
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Cell Aggregation / physiology
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Cell Line, Tumor
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Cell Movement / genetics
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Cell Movement / physiology*
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DNA, Complementary / genetics
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Gene Expression
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Glycoproteins / pharmacology
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Humans
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Intracellular Signaling Peptides and Proteins
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Neoplasm Invasiveness
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Neoplasms / genetics
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Neoplasms / metabolism
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Neoplasms / pathology
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Signal Transduction / drug effects
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Signal Transduction / physiology*
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TRPP Cation Channels / metabolism*
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Transfection
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Wnt1 Protein / antagonists & inhibitors
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Wnt1 Protein / physiology*
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beta Catenin / metabolism
Substances
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Cadherins
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DNA, Complementary
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Glycoproteins
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Intracellular Signaling Peptides and Proteins
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TRPP Cation Channels
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WD repeat containing planar cell polarity effector
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Wnt1 Protein
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beta Catenin
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polycystic kidney disease 1 protein