GATA-binding transcription factors in mast cells regulate the promoter of the mast cell carboxypeptidase A gene

J Biol Chem. 1991 Dec 5;266(34):22948-53.

Abstract

The transcription factors GATA-1, GATA-2, and GATA-3 were found to be expressed in several mouse and rat mast cell lines that contain mast cell carboxypeptidase A (MC-CPA) and other proteases in their cytoplasmic granules. GATA-1 mRNA was not detected in P815 cells, an immature mouse mastocytoma-derived cell line that lacks electron-dense granules and has low levels of secretory granule proteases. Because the 5'-flanking regions of the mouse and human MC-CPA genes contained a conserved GATA-binding motif 51 base pairs upstream of their translation initiation sites, the ability of GATA-binding proteins to regulate the promoter activity of the MC-CPA gene was examined in rat basophilic leukemia cells, mouse P815 cells, and transfected mouse P815 cells that expressed GATA-1. In all three mast cell lines, the promoter activity of the MC-CPA gene depended on the GATA binding site. GATA-1, GATA-2, and GATA-3 are thus the first DNA-binding proteins identified in mast cells which regulate the promoter activity of a gene that encodes a secretory granule protease.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Carboxypeptidases / genetics*
  • Carboxypeptidases / metabolism
  • Carboxypeptidases A
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / metabolism
  • Erythroid-Specific DNA-Binding Factors
  • GATA1 Transcription Factor
  • GATA2 Transcription Factor
  • GATA3 Transcription Factor
  • Gene Expression Regulation, Enzymologic
  • Mast Cells / enzymology*
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic*
  • Rats
  • Trans-Activators / biosynthesis
  • Trans-Activators / metabolism
  • Transcription Factors / biosynthesis
  • Transcription Factors / metabolism*
  • Tumor Cells, Cultured

Substances

  • DNA-Binding Proteins
  • Erythroid-Specific DNA-Binding Factors
  • GATA1 Transcription Factor
  • GATA2 Transcription Factor
  • GATA3 Transcription Factor
  • Gata1 protein, mouse
  • Gata1 protein, rat
  • Gata2 protein, mouse
  • Gata2 protein, rat
  • Gata3 protein, mouse
  • Trans-Activators
  • Transcription Factors
  • Carboxypeptidases
  • Carboxypeptidases A

Associated data

  • GENBANK/M55199
  • GENBANK/M55200
  • GENBANK/M55201
  • GENBANK/M76557
  • GENBANK/M96680
  • GENBANK/M96681
  • GENBANK/M96682
  • GENBANK/S66610
  • GENBANK/S66768
  • GENBANK/S68747