Abstract
The FOG family of transcriptional co-factors is composed of two members in mammals: FOG-1 and FOG-2. Both have been shown to bind to GATA factors and function as transcriptional co-repressors in specific cell and promoter contexts. We have previously defined a novel repression domain localized to the N-terminus of each FOG family member, the FOG repression motif, which is necessary for FOG-mediated transcriptional repression. In this report, we describe the identification and characterization of a novel isoform of FOG-2 lacking the FOG repression motif. In contrast to full-length FOG-2, this isoform is expressed predominately in the embryonic and adult heart. It can bind GATA4 avidly, but is unable to repress GATA4-mediated activation of cardiac-restricted gene promoters. Together, these results suggest that FOG-2 repressive activity may be modulated by the generation of isoforms of FOG-2 lacking the FOG repression motif.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing*
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Animals
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Binding Sites
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Blotting, Western
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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GATA4 Transcription Factor / genetics
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GATA4 Transcription Factor / metabolism
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Gene Expression Profiling
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Male
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Mice
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Molecular Sequence Data
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NIH 3T3 Cells
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Protein Binding
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / isolation & purification
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RNA, Messenger / metabolism
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Repressor Proteins / genetics*
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Repressor Proteins / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Transcriptional Activation
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Transfection
Substances
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DNA-Binding Proteins
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GATA4 Transcription Factor
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Gata4 protein, mouse
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Protein Isoforms
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RNA, Messenger
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Repressor Proteins
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Transcription Factors
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Zfpm2 protein, mouse