Abstract
We demonstrate a novel NMR method for the mapping of protein-protein interaction sites. In our approach protein-protein binding sites are mapped by competition binding experiments using indirect NMR reporter technology and Ala positional scanning. The methodology provides high sensitivity, ease of implementation and high-throughput capabilities. The feasibility of the technique is demonstrated with an application to the beta-Catenin/Tcf4 complex.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Binding, Competitive
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Humans
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Ligands
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Models, Molecular
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Mutation / genetics*
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Nuclear Magnetic Resonance, Biomolecular / methods*
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Protein Interaction Mapping / methods*
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Reproducibility of Results
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TCF Transcription Factors / metabolism
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Transcription Factor 7-Like 2 Protein
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beta Catenin / metabolism
Substances
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Ligands
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TCF Transcription Factors
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TCF7L2 protein, human
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Transcription Factor 7-Like 2 Protein
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beta Catenin