"Home-brew" FISH assay shows higher efficiency than BCR-ABL dual color, dual fusion probe in detecting microdeletions and complex rearrangements associated with t(9;22) in chronic myeloid leukemia

Cancer Genet Cytogenet. 2007 Apr 15;174(2):121-6. doi: 10.1016/j.cancergencyto.2006.09.025.

Abstract

We carried out fluorescence in situ hybridization (FISH) studies on 18 Ph+ chronic myeloid leukemia (CML) cases with chromosome 22 genomic deletions with the Vysis BCR-ABL dual-color/dual-fusion probe (BCR-ABL DC/DF) to compare the hybridization patterns obtained with this approach to those obtained with the "home brew" BAC/PAC system. Our results are the following: chromosome 22 microdeletions less than 400 kilobases (Kb) were not detected by the BCR DC/DF probe; FISH analysis with the BCR DC/DF probe in cases bearing chromosome 22 microdeletions ranging from 400 to 700 Kb produced a faint signal on the der(9); and the BCR-ABL DC/DF FISH pattern was comparable to the one obtained by the home brew probe in the presence of a 900-Kb chromosome 22 microdeletion. Our home-brew FISH system represents an accurate method for revealing a subset of CML patients with der(9) microdeletions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Deletion
  • Chromosomes, Human, Pair 22*
  • Chromosomes, Human, Pair 9*
  • DNA Probes / genetics
  • Fusion Proteins, bcr-abl / genetics*
  • Gene Rearrangement
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
  • Reproducibility of Results
  • Translocation, Genetic*

Substances

  • DNA Probes
  • Fusion Proteins, bcr-abl