Serum levels of the Th1 promoter IL-12 and the Th2 chemokine TARC are elevated in erythema multiforme and Stevens-Johnson syndrome/toxic epidermal necrolysis and correlate with soluble Fas ligand expression. An immunoenzymatic study from the Italian Group of Immunopathology

Dermatology. 2007;214(4):296-304. doi: 10.1159/000100880.

Abstract

Background: No data exist as to Th2 chemokines in erythema multiforme (EM) and Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN).

Objective: To evaluate thymus- and activation-regulated chemokine (TARC), macrophage-derived chemokine (MDC) and regulated upon activation, normal T-lymphocyte-expressed and secreted chemokine (RANTES) expression in EM and SJS/TEN and to correlate with the serum levels of the Th1 promoter interleukin (IL)-12 and soluble Fas ligand (sFasL).

Materials and methods: IL-12, sFasL, TARC, MDC and RANTES expression were analyzed by ELISA techniques in 31 untreated EM (n = 24) or SJS/TEN (n = 7) patients and in 28 healthy donors (HD).

Results: EM and SJS/TEN exhibited significantly higher levels of TARC, IL-12 and sFasL with respect to HD. TARC upregulation paralleled both the IL-12 (p = 0.0225) and sFasL increase (p = 0.0194).

Conclusions: Our results support a role of TARC in the pathophysiology of EM/SJS/TEN and confirm the coexistence of a Th2 response in addition to the predominant Th1 profile.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Chemokine CCL17
  • Chemokine CCL22
  • Chemokine CCL5 / biosynthesis
  • Chemokine CCL5 / blood
  • Chemokines
  • Chemokines, CC / biosynthesis*
  • Chemokines, CC / blood
  • Enzyme-Linked Immunosorbent Assay
  • Erythema Multiforme / immunology*
  • Erythema Multiforme / physiopathology
  • Fas Ligand Protein / biosynthesis*
  • Fas Ligand Protein / blood
  • Female
  • Humans
  • Interleukin-12 / biosynthesis*
  • Interleukin-12 / blood
  • Male
  • Middle Aged
  • Prospective Studies
  • Stevens-Johnson Syndrome / immunology*
  • Stevens-Johnson Syndrome / physiopathology
  • Th1 Cells / immunology
  • Th2 Cells / immunology

Substances

  • CCL17 protein, human
  • CCL22 protein, human
  • Chemokine CCL17
  • Chemokine CCL22
  • Chemokine CCL5
  • Chemokines
  • Chemokines, CC
  • Fas Ligand Protein
  • Interleukin-12