Abstract
The mechanisms that allow antigen-presenting cells (APCs) to selectively present extracellular antigen to CD8+ effector T cells (cross-presentation) or to CD4+ T helper cells are not fully resolved. We demonstrated that APCs use distinct endocytosis mechanisms to simultaneously introduce soluble antigen into separate intracellular compartments, which were dedicated to presentation to CD8+ or CD4+ T cells. Specifically, the mannose receptor supplied an early endosomal compartment distinct from lysosomes, which was committed to cross-presentation. These findings imply that antigen does not require intracellular diversion to access the cross-presentation pathway, because it can enter the pathway already during endocytosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigen Presentation*
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Antigens / immunology*
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CD4-Positive T-Lymphocytes / immunology*
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CD8-Positive T-Lymphocytes / immunology*
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Dendritic Cells / immunology
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Endocytosis
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Endosomes / immunology
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Endosomes / metabolism
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Histocompatibility Antigens Class II / immunology
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Lectins, C-Type / metabolism
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Lymphocyte Activation*
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Lysosomes / immunology
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Lysosomes / metabolism
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Macrophages / immunology
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Mannose Receptor
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Mannose-Binding Lectins / metabolism
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Mice
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Mice, Inbred C57BL
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Models, Immunological
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Ovalbumin / immunology
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Pinocytosis
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Receptors, Cell Surface / metabolism
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Receptors, Scavenger / metabolism
Substances
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Antigens
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Histocompatibility Antigens Class II
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Lectins, C-Type
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Mannose Receptor
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Mannose-Binding Lectins
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Receptors, Cell Surface
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Receptors, Scavenger
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Ovalbumin