Crystallization and preliminary X-ray diffraction data of the Fab fragment of a monoclonal antibody against apamin, a bee venom neurotoxin

C Devaux; M L Defendini; P M Alzari; C Abergel; C Granier; J C Fontecilla-Camps

doi:10.1016/0014-5793(91)80941-u

Crystallization and preliminary X-ray diffraction data of the Fab fragment of a monoclonal antibody against apamin, a bee venom neurotoxin

FEBS Lett. 1991 Jul 29;286(1-2):64-6. doi: 10.1016/0014-5793(91)80941-u.

Authors

C Devaux¹, M L Defendini, P M Alzari, C Abergel, C Granier, J C Fontecilla-Camps

Affiliation

¹ Laboratoire de Biochimie, CNRS URA 1455, Faculté de Médecine-Nord, Marseille, France.

PMID: 1747157
DOI: 10.1016/0014-5793(91)80941-u

Abstract

Fab fragments of anti-apamin monoclonal antibodies have been purified to homogeneity and crystallized. The crystals belong to the monoclinic space group P21 with cell dimensions a = 99.0 +/- 0.3 A, b = 137.1 +/- 0.4 A, c = 76.0 +/- 0.2 A and beta = 92.9 +/- 0.9 degrees. They most likely contain four molecules in the asymmetric unit (Vm = 2.39 A3/Da). The possibility of the existence of non-crystallographic symmetry is discussed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal / chemistry*
Antibodies, Monoclonal / immunology
Antibodies, Monoclonal / isolation & purification
Apamin / immunology*
Crystallization
Electrophoresis
Immunoglobulin Fab Fragments / chemistry*
Immunoglobulin Fab Fragments / immunology
Immunoglobulin Fab Fragments / isolation & purification
X-Ray Diffraction

Substances

Antibodies, Monoclonal
Immunoglobulin Fab Fragments
Apamin