Abstract
SUMMARYNewborn larvae (NBL) and adult (Ad) stage-specifically expressed genes or members of gene families of Trichinella spiralis were identified by suppression subtractive hybridization (SSH). Six cDNA clones were identified as NBL stage-specific, including 1 member of the T. spiralis gene family encoding glutamic acid-rich proteins, 2 clones encoding novel serine proteases, 2 closely related clones encoding proteins that are members of a deoxyribonuclease II (DNase II)-like family and 1 clone with no similarity to known genes. Four stage-specific clones encoding homologues of retinoid X receptor, caveolin, C2H2 type zinc finger protein and a putative protein with no homology to known sequences were obtained from 3-day-old adult worms. One gene specifically up-regulated in the 5-day-old adult worms encoding a putative cuticle collagen was also identified.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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DNA, Complementary / metabolism
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Gene Expression Profiling / veterinary
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Gene Expression Regulation, Developmental*
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Larva / genetics
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Larva / physiology
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Life Cycle Stages / genetics*
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Mice
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Molecular Sequence Data
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Nucleic Acid Hybridization / methods
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Rats
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Trichinella spiralis / genetics*
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Trichinella spiralis / growth & development*
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Trichinella spiralis / metabolism
Associated data
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GENBANK/DQ159094
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GENBANK/EF041131
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GENBANK/EF041132
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GENBANK/EF041133
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GENBANK/EF041134
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GENBANK/EF041135
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GENBANK/EF041136
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GENBANK/EF041137
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GENBANK/EF041138
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GENBANK/EF041139
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GENBANK/EF041140
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GENBANK/EF041141
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GENBANK/EF041142
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GENBANK/EF041143
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GENBANK/EF041144
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GENBANK/EF066629
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GENBANK/EF066630
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GENBANK/EF066631
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GENBANK/EF066632
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GENBANK/EF066633
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GENBANK/EF066634
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GENBANK/EF066635
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GENBANK/EF066636
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GENBANK/EF066637
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GENBANK/EF066638
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GENBANK/EF066639
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GENBANK/EF066640
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GENBANK/EF066641
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GENBANK/EF066642
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GENBANK/EF066643
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GENBANK/EF066644
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GENBANK/EF066645
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GENBANK/EF066646
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GENBANK/EF066647
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GENBANK/EF066648
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GENBANK/EF066649