Synthesis of fluorescently labelled and internally quenched UDP-Gal probes

Frank Schweizer

doi:10.1016/j.carres.2007.03.029

Synthesis of fluorescently labelled and internally quenched UDP-Gal probes

Carbohydr Res. 2007 Sep 3;342(12-13):1831-40. doi: 10.1016/j.carres.2007.03.029. Epub 2007 Apr 11.

Author

Frank Schweizer¹

Affiliation

¹ Department of Chemistry, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2. [email protected]

PMID: 17475229
DOI: 10.1016/j.carres.2007.03.029

Abstract

The preparation of fluorescently labelled and internally quenched UDP-Gal probes bearing a fluorescence emitter and a quencher is described. The rate of transfer using several galactosyltransferases was examined. Our results demonstrate that galactose-modified, sugar-nucleotide-modified and double modified UDP-Gal analogues are recognized as weak substrates by blood group B alpha-(1-->3) galactosyltransferase, alpha-(1-->3) galactosyltransferase and milk bovine beta-(1-->4) galactosyltransferase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Conformation
Fluorescent Dyes
Glycosylation
Indicators and Reagents
Models, Molecular
Nucleotides / chemistry
Oligosaccharides / chemistry
Uridine Diphosphate Galactose / chemistry*

Substances

Fluorescent Dyes
Indicators and Reagents
Nucleotides
Oligosaccharides
Uridine Diphosphate Galactose