PCR amplification of hydrogen cyanide biosynthetic locus hcnAB in Pseudomonas spp

M Svercel; B Duffy; G Défago

doi:10.1016/j.mimet.2007.03.018

PCR amplification of hydrogen cyanide biosynthetic locus hcnAB in Pseudomonas spp

J Microbiol Methods. 2007 Jul;70(1):209-13. doi: 10.1016/j.mimet.2007.03.018. Epub 2007 Apr 6.

Authors

M Svercel¹, B Duffy, G Défago

Affiliation

¹ Plant pathology, Institute of Integrative Biology, ETH-Zürich, CH-8092 Zürich, Switzerland.

PMID: 17475352
DOI: 10.1016/j.mimet.2007.03.018

Abstract

A PCR-based assay targeting hcnAB, essential genes for hydrogen cyanide (HCN) biosynthesis, allowed sensitive detection of HCN(+) pseudomonads between logs 2.9 and 3.5 cells per PCR reaction tube. RFLP analysis revealed 13 allele combinations among selected 2,4-diacetylphloroglucinol-producing (Phl(+))HCN(+), and 13 alleles in Phl(-) HCN(+) strains from a global collection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Fingerprinting
DNA, Bacterial / genetics
Genes, Bacterial*
Genotype
Hydrogen Cyanide / metabolism*
Metabolic Networks and Pathways / genetics*
Polymerase Chain Reaction / methods*
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Pseudomonas / classification*
Pseudomonas / genetics*
Pseudomonas / isolation & purification
Pseudomonas / metabolism

Substances

DNA, Bacterial
Hydrogen Cyanide