Abstract
The esrB gene of Edwardsiella tarda, which encodes a regulator protein of the type III secretion system, was mutated by the unmarked deletion method and reintroduced by allelic exchange into the chromosome of E. tarda LSE40 by means of the suicide vector pRE112. The LSE40 esrB mutant was highly attenuated when inoculated intraperitoneally into turbot Scophthamus maximus L., showing a 50% lethal dose of 10(8.1)cfu/fish. The esrB mutants were not recoverable from the internal organs at 14 days post-inoculation. Vaccination with a single dose of 10(5)-10(7) cfu/fish of the esrB mutant elicited significant protection against the wild-type strain of E. tarda LSE40 (relative percentage survival>50%). The protection correlated well with the antibody titres in the serum of vaccinated fish.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Bacterial / blood
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Bacterial Proteins / genetics
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Bacterial Proteins / immunology*
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Bacterial Vaccines / administration & dosage
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Bacterial Vaccines / immunology*
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Edwardsiella tarda / genetics*
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Edwardsiella tarda / immunology*
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Edwardsiella tarda / pathogenicity
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Enterobacteriaceae Infections / immunology
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Enterobacteriaceae Infections / mortality
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Enterobacteriaceae Infections / prevention & control
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Enterobacteriaceae Infections / veterinary*
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Fish Diseases / immunology
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Fish Diseases / microbiology
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Fish Diseases / mortality
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Fish Diseases / prevention & control
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Flatfishes / immunology*
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Hemorrhagic Septicemia / mortality
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Hemorrhagic Septicemia / prevention & control
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Hemorrhagic Septicemia / veterinary*
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Mutation / genetics
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Survival Analysis
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Time Factors
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Vaccines, Attenuated / administration & dosage
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Vaccines, Attenuated / immunology
Substances
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Antibodies, Bacterial
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Bacterial Proteins
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Bacterial Vaccines
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Vaccines, Attenuated